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Cloud-clone

SKU:RPB978Hu02

Recombinant Complement Factor I (CFI)

Recombinant Complement Factor I (CFI)

Regular price $430.00 USD
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UOM
Purity
Organism species

Prokaryotic expression

Product No.

RPB978Hu02

Organism Species

Homo sapiens (Human).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

28.0kDa

Accurate Molecular Mass

28kDa(Analysis of differences refer to the manual)

Residues & Tags

Leu28~Gly243 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

8.0

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
J Thromb Haemost A case report of thrombotic microangiopathy without renal involvement carrying two novel mutations in complement-regulator genes PubMed: 26613809
Pediatr Int Atypical hemolytic uremic syndrome: Korean pediatric series PubMed: 25443527
United States Patent Application  NOVEL ASSAY y2016:139118
Researchgate Compliment factor i bioassay US20170285024
PLoS One A monoclonal antibody targeting amyloid β (Aβ) restores complement factor I bioactivity: Potential implications in age-related macular degeneration and … Pubmed:29782502
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H 33788378
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