Cloud-clone
SKU:RPB430Ra01
Recombinant Semaphorin 4D (SEMA4D)
Recombinant Semaphorin 4D (SEMA4D)
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Prokaryotic expression
Product No.
RPB430Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Cytoplasm, Extracellular matrix
Predicted Molecular Mass
68.9kDa
Accurate Molecular Mass
69kDa(Analysis of differences refer to the manual)
Residues & Tags
Leu145~Gly494 with N-terminal His and GST Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
7.2
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Expert Opin Ther Targets. | Circulating semaphorin-4D and plexin-B1 levels in postmenopausal women with low bone mass: the 3-month effect of zoledronic acid, denosumab or teriparatide treatment Pubmed:25395071 |
| Obesity (Silver Spring). | Genome-wide analysis identifies an african-specific variant in SEMA4D associated with body mass index. pubmed:28296344 |
| Clinical Lymphoma, Myeloma & Leukemia | A Revised Staging System for Waldenström's Macroglobulinemia 10.1016/j.clml.2017.07.146 |
| Clinical Lymphoma, Myeloma & Leukemia | Extramedullary Multiple Myeloma: Clinical Features and Prognosis in Argentina 10.1016/j.clml.2017.07.144 |
| Blood Cancer Journal | Semaphorin 4D correlates with increased bone resorption, hypercalcemia, and disease stage in newly diagnosed patients with multiple myeloma Pubmed:29748532 |
| Analytical Biochemistry | A high-sensitivity enzyme immunoassay for the quantification of soluble human semaphorin 4D in plasma Pubmed: 30879960 |
| Journal of Allergy and Clinical Immunology-In Practice | Pathological and therapeutic implications of eosinophil-derived semaphorin 4D in eosinophilic chronic rhinosinusitis Pubmed: 32035658 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB430Ra01 | Recombinant Semaphorin 4D (SEMA4D) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB430Ra01 | Polyclonal Antibody to Semaphorin 4D (SEMA4D) | WB; IHC; ICC; IP. |
