Cloud-clone
SKU:RPB870Hu01
Recombinant Tubulin Beta (TUBb)
Recombinant Tubulin Beta (TUBb)
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Prokaryotic expression
Product No.
RPB870Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Cytoplasm
Predicted Molecular Mass
32.1kDa
Accurate Molecular Mass
32kDa(Analysis of differences refer to the manual)
Residues & Tags
Val170~Val419 with N-terminal His Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
6.6
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| archives of pharmacal research | Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues pubmed:27747473 |
| archiv der pharmazie (Weinheim). | New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition. pubmed:28150327 |
| ACS Applied Materials & Interfaces | Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixture pubmed:28112513 |
| Future Medicinal Chemistry | Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agents Pubmed:29779400 |
| Future Medicinal Chemistry | Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerization Pubmed:29966433 |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids Pubmed: 30237167 |
| Journal of Enzyme Inhibition and Medicinal Chemistry | Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2Inhibitors Pubmed: 30191744 |
| European Journal of Medicinal Chemistry | Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking study Pubmed: 31536891 |
| ACS Sensors | Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platform Pubmed: 32207606 |
| Bioorganic Chemistry | Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their … Pubmed: 32325332 |
| BRITISH JOURNAL OF NUTRITION | Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats Pubmed: 33028437 |
| European Journal of Medicinal Chemistry | Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon … Pubmed: 33158579 |
| J BIOMOL STRUCT DYN/JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | Cytotoxicity, in silico predictions and molecular studies for androstane heterocycle compounds revealed potential antitumor agent against lung cancer cells 33300466 |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212 |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells 34837026 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APB870Hu01 | Active Tubulin Beta (TUBb) | Cell culture; Activity Assays. |
| RPB870Hu01 | Recombinant Tubulin Beta (TUBb) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAB870Hu01 | Anti-Tubulin Beta (TUBb) Polyclonal Antibody | Loading Control of WB. |
| PAB870Hu01 | Polyclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu22 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu23 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu25 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu27 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu24 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu21 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| CAB870Hu22 | Anti-Tubulin Beta (TUBb) Monoclonal Antibody | Loading Control of WB. |
