Eukaryotic Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)

Eukaryotic expression

Product No.

EPC300Hu61

Organism Species

Homo sapiens (Human).

Source

Eukaryotic expression

Host

293F cell

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

19.8kDa

Accurate Molecular Mass

21kDa(Analysis of differences refer to the manual)

Residues & Tags

Leu25~Leu182 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 97%

Isoelectric Point

8.4

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.