Active Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF)

Figure 1. Cell proliferation of ECV-304 cells after stimulated with EG-VEGF.
Endothelial gland-derived VEGF (EG-VEGF) is an angiogenic protein that is structurally unrelated to VEGF. It is expressed in steroidogenic tissues such as adrenal gland, ovary, testis, and placenta. Like VEGF it can induce fenestrae in endothelial cells. To test the effect of EG-VEGF on cell proliferation of ECV-304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of EG-VEGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37℃.
(A) Unstimulated ECV-304 cells cultured in 1640 for 96h;
(B) ECV-304 cells cultured in 1640, stimulated with 10ng/mL VEGF121 for 96h.

Figure. Cell proliferation of ECV-304 cells after stimulated with EG-VEGF