Cloud-clone
SKU:RPC156Si01
Recombinant Collagen Type X (COL10)
Recombinant Collagen Type X (COL10)
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Prokaryotic expression
Product No.
RPC156Si01
Organism Species
Rhesus monkey (Simian).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
16.4kDa
Accurate Molecular Mass
16kDa(Analysis of differences refer to the manual)
Residues & Tags
Thr547~Met680 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
6.7
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Tissue Eng Part A | Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I PubMed: 26123891 |
| Gene Ther | rAAV-mediated overexpression of sox9, TGF-β and IGF-I in minipig bone marrow aspirates to enhance the chondrogenic processes for cartilage repair PubMed: 26583804 |
| American Journal of Pathology | Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture Healing Pubmed: 30339839 |
| Type X Collagen Assays and Methods of Use Thereof |
| Catalog No. | Related products for research use of Rhesus monkey (Simian) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC156Si01 | Recombinant Collagen Type X (COL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
