Recombinant Permeability Glycoprotein (Pgp)

Prokaryotic expression

Product No.

RPB690Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Membrane, Secreted

Predicted Molecular Mass

33.8kDa

Accurate Molecular Mass

34kDa(Analysis of differences refer to the manual)

Residues & Tags

Ala999~Ser1277 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

9.1

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
International Forum of Allergy & Rhinology	P-glycoprotein promotes epithelial T helper 2–associated cytokine secretion in chronic sinusitis with nasal polyps Onlinelibrary: alr.21316
International Forum of Allergy & Rhinology	P‐glycoprotein regulates Staphylococcus aureus enterotoxin B–stimulated interleukin‐5 and thymic stromal lymphopoietin secretion in organotypic mucosal explants PubMed: 26625351
American Journal of Rhinology & Allergy	Intact soluble P-glycoprotein is secreted by sinonasal epithelial cells. pubmed:27456593
The Laryngoscope	Secreted P-glycoprotein is a noninvasive biomarker of chronic rhinosinusitis pubmed:27577924
Materials Science and Engineering: C	Design of colloidal drug carriers of celecoxib for use in treatment of breast cancer and leukemia
Molecular Cancer	Chemotherapeutic drugs stimulate the release and recycling of extracellular vesicles to assist cancer cells in developing an urgent chemoresistance Pubmed: 31830995

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPB690Ra01	Recombinant Permeability Glycoprotein (Pgp)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB690Ra01	Polyclonal Antibody to Permeability Glycoprotein (Pgp)	WB; IHC; ICC; IP.
SEB690Ra	ELISA Kit for Permeability Glycoprotein (Pgp)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB690Ra	Magnetic Luminex Assay Kit for Permeability Glycoprotein (Pgp) ,etc.	Magnetic Luminex Assay for Antigen Detection.