Recombinant Bone Morphogenetic Protein 4 (BMP4)

Prokaryotic expression

Product No.

RPA014Fi01

Organism Species

Danio rerio (Zebrafish).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

46.8kDa

Accurate Molecular Mass

45kDa(Analysis of differences refer to the manual)

Residues & Tags

Ser25~Arg400 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

6.9

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Journal of Cellular Biochemistry	Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway PubMed: 26681207
Domestic Animal Endocrinology	Expression analysis of bone morphogenetic protein 4 between fat and lean birds in adipose tissue and serum Pubmed:26945137
FEBS Open Bio	Bone morphogenetic protein 4 (BMP 4) regulates immortalized chicken preadipocyte proliferation by promoting G1/S cell cycle progression Pubmed: 30972973
Arch Gynecol Obstet	The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese … 34417839

Catalog No.	Related products for research use of Danio rerio (Zebrafish) Organism species	Applications (RESEARCH USE ONLY!)
RPA014Fi01	Recombinant Bone Morphogenetic Protein 4 (BMP4)	Positive Control; Immunogen; SDS-PAGE; WB.