Recombinant Histone H3 (H3)

Prokaryotic expression

Product No.

RPA285Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Nucleus, Chromosome

Predicted Molecular Mass

16.67 kDa

Accurate Molecular Mass

18 kDa(Analysis of differences refer to the manual)

Residues & Tags

Met1~Ala136 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Traits

Freeze-dried powder

Purity

> 95%

Isoelectric Point

11.7

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Biomed Res Int	A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649890
Cell Death & Disease	Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death Pubmed:35732625

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPA285Ra01	Recombinant Histone H3 (H3)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA285Ra01	Polyclonal Antibody to Histone H3 (H3)	WB; IHC; ICC; IP.
KSA285Ra01	ELISA Kit DIY Materials for Histone H3 (H3)	Main materials for "Do It (ELISA Kit) Yourself".