Recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)

Prokaryotic expression

Product No.

RPA045Ov01

Organism Species

Ovis aries; Ovine (Sheep).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

44.4kDa

Accurate Molecular Mass

51kDa(Analysis of differences refer to the manual)

Residues & Tags

Ala18~Lys144 with N-terminal His and GST Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

5.1

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Journal of Toxicology and Environmental Health	Effects of 900-MHz Microwave Radiation on γ-Ray-Induced Damage to Mouse Hematopoietic System PubMed: 20391130
5	Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4×: a phase 2 randomised, double-blind, placebo-controlled trial Pubmed:24525316
Archives of Virology	Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China Pubmed:29305646
BioMed Research International	Activated Macrophages of Monocytic Origin Predominantly Express Proinflammatory Cytokine Genes, Whereas Kupffer Cells Predominantly Express Anti …
bulletin of experimental biology and medicine	Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin Pubmed: 31183654
Exp Mol Pathol	The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡­ 33285209
Vaccine	Co-delivery of PSMA antigen epitope and mGM-CSF with a cholera toxin-like chimeric protein suppressed prostate tumor growth via activating dendritic cells and?¡­ 33612342

Catalog No.	Related products for research use of Ovis aries; Ovine (Sheep) Organism species	Applications (RESEARCH USE ONLY!)
APA045Ov01	Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)	Cell culture; Activity Assays.
RPA045Ov01	Recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)	Positive Control; Immunogen; SDS-PAGE; WB.