Recombinant Cytokeratin 4 (CK4)

Prokaryotic expression

Product No.

RPA489Mu01

Organism Species

Mus musculus (Mouse).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

16.3kDa

Accurate Molecular Mass

16kDa(Analysis of differences refer to the manual)

Residues & Tags

Asp194~Val301 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 95%

Isoelectric Point

4.7

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Molecular Medicine Reports	Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes Pubmed:29749501
FEBS Open Bio	Development of a novel murine heart failure model overexpressing human renin and angiotensinogen Pubmed: 32056390
J Clin Periodontol	Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal … Pubmed:35451104

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPA489Mu01	Recombinant Cytokeratin 4 (CK4)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA489Mu01	Polyclonal Antibody to Cytokeratin 4 (CK4)	WB; IHC; ICC; IP.