Recombinant Lipase, Hormone Sensitive (LIPE)

Prokaryotic expression

Product No.

RPB276Mu01

Organism Species

Mus musculus (Mouse).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Membrane, Cytoplasm

Predicted Molecular Mass

15.6kDa

Accurate Molecular Mass

16kDa(Analysis of differences refer to the manual)

Residues & Tags

Gln652~Asn759 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

8.0

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Journal of Functional Foods	Polysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet 10.1016/j.jff.2017.07.020
Journal of Lipid Research	Zinc-α2-glycoprotein alleviates high fat diet-induced insulin resistance accompanied with the decreased lipid depot in skeletal muscle in mice Pubmed: 30318474
	Effect of Carbonate Apatite Membrane as Adjunctive Therapy of Scaling and Root Planing on Gingival Crevicular Fluid Matrix Metalloproteinase-8 in Chronic …

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPB276Mu01	Recombinant Lipase, Hormone Sensitive (LIPE)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB276Mu01	Polyclonal Antibody to Lipase, Hormone Sensitive (LIPE)	WB; IHC; ICC; IP.
SEB276Mu	ELISA Kit for Lipase, Hormone Sensitive (LIPE)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB276Mu	Magnetic Luminex Assay Kit for Lipase, Hormone Sensitive (LIPE) ,etc.	Magnetic Luminex Assay for Antigen Detection.