Recombinant Apolipoprotein E (APOE)

Prokaryotic expression

Product No.

RPA704Bo01

Organism Species

Bos taurus; Bovine (Cattle).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

37.8kDa

Accurate Molecular Mass

40kDa(Analysis of differences refer to the manual)

Residues & Tags

Asp19~His316 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

5.4

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Seizure - European Journal of Epilepsy	Cerebrospinal fluid apolipoprotein E concentration decreases after seizure Seizure: PIIS1059131109002441
Biochimica et Biophysica Acta	Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties PubMed: 23328279
Medical Oncology	Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma Pubmed: 23609192
Diabetes.	Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity Pubmed:25249572
renal failure	Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study PubMed: 25707516
Biochim Biophys Acta	Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism PubMed: 26515253
Diabetes	Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity PubMed: 25249572
Scientific Reports	Adaptive immunity against gut microbiota enhances apoE-mediated immune regulation andreduces atherosclerosis and western-diet-related inflammation. pubmed:27383250
Biochemical Pharmacology	Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice Pubmed:30026023
Oncotarget	Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse … Pubmed:29731975
Proteomics Clinical Applications	Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy Pubmed: 31162828
Nature Communications	Hepatic HuR modulates lipid homeostasis in response to high-fat diet Pubmed: 32546794
VETERINARY PATHOLOGY	Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats Pubmed: 32880234
Journal of Clinical Medicine	The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome 33321701
Cancer Cell Int	Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer 33602229
Nat Commun	GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity 34853313
Talanta	Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled … Pubmed:35378356
Analytica Chimica Acta	Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal … Pubmed:35361429

Catalog No.	Related products for research use of Bos taurus; Bovine (Cattle) Organism species	Applications (RESEARCH USE ONLY!)
RPA704Bo01	Recombinant Apolipoprotein E (APOE)	Positive Control; Immunogen; SDS-PAGE; WB.