Recombinant Staphylococcal Protein A (SPA)

Prokaryotic expression

Product No.

RPA169Ge01

Organism Species

Pan-species (General).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

35.46kDa

Accurate Molecular Mass

35kDa(Analysis of differences refer to the manual)

Residues & Tags

Thr31~Pro334 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

4.5

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Respiratory research	Effects and molecular mechanisms of intrauterine infection/inflammation on lung development Pubmed:29747649

Catalog No.	Related products for research use of Pan-species (General) Organism species	Applications (RESEARCH USE ONLY!)
RPA169Ge01	Recombinant Staphylococcal Protein A (SPA)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA169Ge02	Recombinant Staphylococcal Protein A (SPA)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA169Ge03	Recombinant Staphylococcal Protein A (SPA)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA169Ge04	Recombinant Staphylococcal Protein A (SPA)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA169Ge01	Polyclonal Antibody to Staphylococcal Protein A (SPA)	WB; IHC; ICC; IP.
LAA169Ge91	HRP-Linked Polyclonal Antibody to Staphylococcal Protein A (SPA)	WB; IHC; ICC.