Recombinant Granzyme A (GZMA)

Prokaryotic expression

Product No.

RPA599Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Nucleus, Cytoplasm, Extracellular matrix

Predicted Molecular Mass

55.8kDa

Accurate Molecular Mass

59kDa(Analysis of differences refer to the manual)

Residues & Tags

Glu27~Val261 with N-terminal His and GST Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

9.4

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine	Citations
Cell Reports	Granzyme A Produces Bioactive IL-1β through a Nonapoptotic Inflammasome-Independent Pathway Pubmed:25437548
Journal of Dermatological Science	Relationships among plasma granzyme B level, pruritus and dermatitis in patients with atopicdermatitis. pubmed:27686401
Journal of Maternal-Fetal & Neonatal Medicine	Extracellular granzyme A in amniotic fluid is elevated in the presence of sterile intra-amniotic inflammation in preterm prelabor rupture of membranes Pubmed: 32912008

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPA599Ra01	Recombinant Granzyme A (GZMA)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA599Ra01	Polyclonal Antibody to Granzyme A (GZMA)	WB; IHC; ICC; IP.