Eukaryotic Neutrophil Elastase (NE)

Eukaryotic expression

Product No.

EPA181Mu61

Organism Species

Mus musculus (Mouse).

Source

Eukaryotic expression

Host

293F Cell

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Cytoplasm, Extracellular matrix

Predicted Molecular Mass

21.7kDa

Accurate Molecular Mass

33kDa(Analysis of differences refer to the manual)

Residues & Tags

Ser27~Asn265 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 97%

Isoelectric Point

9.6

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine	Citations
Mediators of Inflammation	Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation Hindawi: 612848
Journal of Photochemistry and Photobiology B: Biology	A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins Pubmed:25732262
International Journal of Molecular Medicine	The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats pubmed:27430552
European Journal of Trauma and Emergency	Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. pubmed:28070608
Clin Exp Pharmacol Physiol.	Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688
Journal of Cardiology	Targeted regulation of sympathetic activity in paraventricular nucleus reduces inducible ventricular arrhythmias in rats after myocardial infarction 10.1016:j.jjcc.2018.06.003
Neuroscience	Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats Pubmed:29684507
Molecular and Cellular Biochemistry	Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes Pubmed:28948423
Neuropsychiatric disease and treatment	The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic … Pubmed:29379286
Behavioural Brain Research	Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats Pubmed: 30423388
Journal of Pineal Research	Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice Pubmed: 30929267
Fundamental & clinical pharmacology	Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo Pubmed: 31313350
Cell Death & Disease	Erythromycin suppresses neutrophil extracellular traps in smoking-related chronic pulmonary inflammation Pubmed: 31515489
Journal of nutritional science and vitaminology	Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively … Pubmed: 31474682
Journal of Clinical Immunology	MiR-608 Exerts Anti-inflammatory Effects by Targeting ELANE in Monocytes Pubmed: 31749032
Pathology & Oncology Research	Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer Pubmed: 31656990
Bioscience Reports	Inhibition of microRNA-146a attenuated heart failure in myocardial infarction rats Pubmed: 31763669
Oxidative Medicine and Cellular Longevity	Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation Pubmed: 31871555
Cytokine	Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460
Journal of neural transmission (Vienna, Austria : 1996)	Neurotransmitter system aberrations in patients with drug addiction Pubmed: 32804296
experimental eye research	Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis Pubmed: 32376471
CLINICAL AND EXPERIMENTAL HYPERTENSION	Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats Pubmed: 32349626
Br J Pharmacol	Macrophage Extracellular Traps Aggravate Iron Overload©\Related Liver Ischemia/Reperfusion Injury 33959955
Evidence-Based Complementary and Alternative Medicine	Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling?¡­ 33505502
Nat Sci Sleep	Depression of Non-Neuronal Cholinergic System May Play a Role in Co-Occurrence of Subjective Daytime Sleepiness and Hypertension in Patients with … 34934375
Neuromodulation: Technology at the Neural Interface	Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome Pubmed:35088760
	A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
APA181Mu61	Active Neutrophil Elastase (NE)	Cell culture; Activity Assays.
RPA181Mu01	Recombinant Neutrophil Elastase (NE)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA181Mu02	Recombinant Neutrophil Elastase (NE)	Positive Control; Immunogen; SDS-PAGE; WB.
EPA181Mu61	Eukaryotic Neutrophil Elastase (NE)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA181Mu01	Polyclonal Antibody to Neutrophil Elastase (NE)	WB; IHC; ICC; IP.
PAA181Mu02	Polyclonal Antibody to Neutrophil Elastase (NE)	WB; IHC; ICC; IP.
SEA181Mu	ELISA Kit for Neutrophil Elastase (NE)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA181Mu	Magnetic Luminex Assay Kit for Neutrophil Elastase (NE) ,etc.	Magnetic Luminex Assay for Antigen Detection.