Eukaryotic Complement Component 5a (C5a)

Eukaryotic expression

Product No.

EPA388Mu61

Organism Species

Mus musculus (Mouse).

Source

Eukaryotic expression

Host

293F Cell

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

10.5kDa

Accurate Molecular Mass

14kDa(Analysis of differences refer to the manual)

Residues & Tags

Asn679~Arg755 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 95%

Isoelectric Point

9.3

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine	Citations
Molecular Immunology	Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297
Journal of Reproductive Immunology	Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845
The Journal of Clinical Investigation	Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis PubMed: PMC3195471
Journal of Antimicrobial Chemotherapy	Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587
Journal of Orthopaedic Research	Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model Pubmed: 23070742
SpringerPlus	Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977
American Journal fo Reproductive Immunology	Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia Pubmed:25209155
Gynecol Obstet Invest	Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247
Journal of Neuroimmunology	Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358
International Immunopharmacology	Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602
Digital Repository	Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183
Environmental Pollution	Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia pubmed:27511439
Cancer Letters	Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression pubmed:29031586
Acta Paediatrica	Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children DOI: 10.1111/apa.14106
Scientific Reports	Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis Pubmed: 30291296
Nutrients	The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479
Journal of NeuroVirology	Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976
PLoS One	Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004
Frontiers in Immunology	C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis Pubmed: 31447855
Phytomedicine	Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 …
Aging	C5aR deficiency attenuates the breast cancer development via the p38/p21 axis Pubmed: 32669478
STEM CELL REVIEWS AND REPORTS	A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … Pubmed: 32406006
	Modulation of C5a-C5aR interactions against murine mammary cancer cell line
FASEB J	C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPA388Mu01	Recombinant Complement Component 5a (C5a)	Positive Control; Immunogen; SDS-PAGE; WB.
APA388Mu61	Active Complement Component 5a (C5a)	Cell culture; Activity Assays.
EPA388Mu61	Eukaryotic Complement Component 5a (C5a)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA388Mu01	Polyclonal Antibody to Complement Component 5a (C5a)	WB; IHC; ICC; IP.
LAA388Mu71	Biotin-Linked Polyclonal Antibody to Complement Component 5a (C5a)	WB; IHC; ICC.
MAA388Mu21	Monoclonal Antibody to Complement Component 5a (C5a)	WB; IHC; ICC; IP.
LAA388Mu72	Biotin-Linked Monoclonal Antibody to Complement Component 5a (C5a)	WB; IHC; ICC.
SEA388Mu	ELISA Kit for Complement Component 5a (C5a)	Enzyme-linked immunosorbent assay for Antigen Detection.
MEA388Mu	Mini Samples ELISA Kit for Complement Component 5a (C5a)	Enzyme-linked immunosorbent assay for Antigen Detection.
HEA388Mu	High Sensitive ELISA Kit for Complement Component 5a (C5a)	Enzyme-linked immunosorbent assay for Antigen Detection.
SCA388Mu	CLIA Kit for Complement Component 5a (C5a)	Chemiluminescent immunoassay for Antigen Detection.
LMA388Mu	Magnetic Luminex Assay Kit for Complement Component 5a (C5a) ,etc.	Magnetic Luminex Assay for Antigen Detection.
PSA388Mu01	Antibody Pair for Complement Component 5a (C5a)	ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA388Mu01	ELISA Kit DIY Materials for Complement Component 5a (C5a)	Main materials for "Do It (ELISA Kit) Yourself".