Recombinant Vinculin (VCL)

Prokaryotic expression

Product No.

RPB839Hu02

Organism Species

Homo sapiens (Human).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Membrane, Cytoplasm

Predicted Molecular Mass

56.6kDa

Accurate Molecular Mass

57kDa(Analysis of differences refer to the manual)

Residues & Tags

Val3~Arg485 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

5.7

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
PBMCs S-NO proteomic signature in heart failure	S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure journals:1384523
J Cell Sci	PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signals Pubmed:35044463

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB839Hu01	Recombinant Vinculin (VCL)	Positive Control; Immunogen; SDS-PAGE; WB.
RPB839Hu02	Recombinant Vinculin (VCL)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB839Hu01	Polyclonal Antibody to Vinculin (VCL)	WB; IHC; ICC; IP.
CAB839Hu01	Anti-Vinculin (VCL) Polyclonal Antibody	Loading Control of WB.
LAB839Hu71	Biotin-Linked Polyclonal Antibody to Vinculin (VCL)	WB; IHC; ICC.
MAB839Hu22	Monoclonal Antibody to Vinculin (VCL)	WB; IHC; ICC; IP.
MAB839Hu21	Monoclonal Antibody to Vinculin (VCL)	WB; IHC; ICC; IP.
CAB839Hu22	Anti-Vinculin (VCL) Monoclonal Antibody	Loading Control of WB
SEB839Hu	ELISA Kit for Vinculin (VCL)	Enzyme-linked immunosorbent assay for Antigen Detection.
AEB839Hu	ELISA Kit for Anti-Vinculin Antibody (Anti-VCL)	Enzyme-linked immunosorbent assay for Antibody Detection.
LMB839Hu	Magnetic Luminex Assay Kit for Vinculin (VCL) ,etc.	Magnetic Luminex Assay for Antigen Detection.