Eukaryotic Coagulation Factor XII (F12)

Eukaryotic expression

Product No.

EPA677Mu61

Organism Species

Mus musculus (Mouse).

Source

Eukaryotic expression

Host

293F Cell

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

65.3kDa

Accurate Molecular Mass

110kDa(Analysis of differences refer to the manual)

Residues & Tags

Ala20~Ser597 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 95%

Isoelectric Point

6.9

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Perfusion	Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing PubMed: 26243277

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
EPA677Mu61	Eukaryotic Coagulation Factor XII (F12)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA677Mu02	Recombinant Coagulation Factor XII (F12)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA677Mu01	Recombinant Coagulation Factor XII (F12)	Positive Control; Immunogen; SDS-PAGE; WB.
APA677Mu61	Active Coagulation Factor XII (F12)	Cell culture; Activity Assays.
PAA677Mu01	Polyclonal Antibody to Coagulation Factor XII (F12)	WB; IHC; ICC; IP.
PAA677Mu02	Polyclonal Antibody to Coagulation Factor XII (F12)	WB; IHC; ICC; IP.
SEA677Mu	ELISA Kit for Coagulation Factor XII (F12)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA677Mu	Magnetic Luminex Assay Kit for Coagulation Factor XII (F12) ,etc.	Magnetic Luminex Assay for Antigen Detection.