Recombinant Heat Shock 70kDa Protein 5 (HSPA5)

Prokaryotic expression

Product No.

RPC343Hu02

Organism Species

Homo sapiens (Human).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

96.5kDa

Accurate Molecular Mass

96kDa(Analysis of differences refer to the manual)

Residues & Tags

Glu19~Leu654 with N-terminal GST Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

4.7

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
41	Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. PubMed: 20522592
pharmacological research	Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors Pubmed: 31048034
EPILEPSY & BEHAVIOR	Fenfluramine acts as a positive modulator of sigma-1 receptors Pubmed: 32169824
Journal of Oncology	Using Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer 33381181
Heliyon	Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior
Medicine (Baltimore)	Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients 34414940

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC343Hu01	Recombinant Heat Shock 70kDa Protein 5 (HSPA5)	Positive Control; Immunogen; SDS-PAGE; WB.
RPC343Hu02	Recombinant Heat Shock 70kDa Protein 5 (HSPA5)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC343Hu01	Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)	WB; IHC; ICC; IP.
MAC343Hu22	Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)	WB; IHC; ICC; IP.
SEC343Hu	ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC343Hu	Magnetic Luminex Assay Kit for Heat Shock 70kDa Protein 5 (HSPA5) ,etc.	Magnetic Luminex Assay for Antigen Detection.