Recombinant Estrogen Receptor Alpha (ERa)

Prokaryotic expression

Product No.

RPB050Hu02

Organism Species

Homo sapiens (Human).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Membrane, Nucleus, Cytoplasm

Predicted Molecular Mass

32.7kDa

Accurate Molecular Mass

33kDa(Analysis of differences refer to the manual)

Residues & Tags

Met297~Ser554 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

7.0

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
The Journal of Steroid Biochemistry and Molecular Biology	Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling Pubmed:24846829
Biochem Biophys Res Commun	Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas PubMed: 26529545
Journal of Environmental Sciences	Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts science:S1001074216300924
cellular microbiology	Influenza A virus NS1 protein-induced JNK activation and apoptosis are not functionally linked. pubmed:28076660
Journal of Steroid Biochemistry and Molecular Biology	Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910
Cellular Physiology and Biochemistry	Antiestrogenic Activity of the Xi-Huang Formula for Breast Cancer by Targeting the Estrogen Receptor α Pubmed:29975948
Carleton University Research Virtual Environment	In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts
Clinical Breast Cancer	Vitamin D receptor in breast cancer tissues and its relation to estrogen receptor alpha (ER-a) gene expression and serum 25-hydroxyvitamin D levels in Egyptian  Doi: 10.1016/j.clbc.2018.12.019
Journal of geriatric psychiatry and neurology	ERα Gene Promoter Methylation in Cognitive Function and Quality of Life of Patients With Alzheimer Disease Pubmed: 30947592
mBio	The Effector Domain of the Influenza A Virus Nonstructural Protein NS1 Triggers Host Shutoff by Mediating Inhibition and Global Deregulation of Host Transcription … 34488451
Cardiometry	Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB050Hu02	Recombinant Estrogen Receptor Alpha (ERa)	Positive Control; Immunogen; SDS-PAGE; WB.
RPB050Hu01	Recombinant Estrogen Receptor Alpha (ERa)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB050Hu01	Polyclonal Antibody to Estrogen Receptor Alpha (ERa)	WB; IHC; ICC; IP.
MAB050Hu22	Monoclonal Antibody to Estrogen Receptor Alpha (ERa)	WB; IHC; ICC; IP.
SEB050Hu	ELISA Kit for Estrogen Receptor Alpha (ERa)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB050Hu	Magnetic Luminex Assay Kit for Estrogen Receptor Alpha (ERa) ,etc.	Magnetic Luminex Assay for Antigen Detection.