Recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL)

Prokaryotic expression

Product No.

RPB091Bo01

Organism Species

Bos taurus; Bovine (Cattle).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

39.2kDa

Accurate Molecular Mass

42kDa(Analysis of differences refer to the manual)

Residues & Tags

Leu18~Arg335 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

7.7

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Journal of Materials Science: Materials in Medicine	Osteopenic bone cell response to strontium-substituted hydroxyapatite SpringerLink: n8233337w171v451
Osteoporosis International	Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activation PubMed: 23400250
Journal of Applied Toxicology	Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source
Acta Pharmacologica Sinica	The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model Pubmed:25544359
Biomedical Materials	Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regeneration Pubmed:25586067
Pharmacology	Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861
J Biomed Mater Res A.	New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization. Pubmed:27239050
J Bone Miner Metab	MiR-142-5p promotes bone repair by maintaining osteoblast activity Pubmed:27085967
Journal of Biomedical Materials Research	New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … doi:10.1002
Journal of Bone and Mineral Metabolism	MiR‑142‑5p promotes bone repair by maintaining osteoblast activity pubmed:27085967
Scientific Reports	Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasma Pubmed:30038263
International Journal of Molecular Sciences	Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo Pubmed:29562730
Pharmacology	Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457
Experimental and Therapeutic Medicine	Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice 10.3892:etm.2018.6353
American Journal of Translational Research	Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9
	The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro
International Journal of Biomaterials	Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect 34104195

Catalog No.	Related products for research use of Bos taurus; Bovine (Cattle) Organism species	Applications (RESEARCH USE ONLY!)
RPB091Bo01	Recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL)	Positive Control; Immunogen; SDS-PAGE; WB.