Recombinant Tryptase (TPS)

Prokaryotic expression

Product No.

RPB070Po02

Organism Species

Sus scrofa; Porcine (Pig).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

30.7kDa

Accurate Molecular Mass

33kDa(Analysis of differences refer to the manual)

Residues & Tags

Ile31~Ser275 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Traits

Freeze-dried powder

Purity

> 95%

Isoelectric Point

6.0

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Journal of Allergy and Clinical Immunology	Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps PubMed: 22534535
J Pediatr.	Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2 Pubmed:24530123
Mediators of Inflammation	Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis Pubmed:25400334
PLOS ONE	A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats Pubmed:25541997
Neurogastroenterology & Motility	Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery Pubmed:25677271
Mediators Inflamm	Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report PubMed: 26089601
Int Immunopharmacol	Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis PubMed: 26049029
Mediators of Inflammation	Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins Journals: Mi
Mediators of Inflammation	Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins Pubmed:27057098
Analytica Chimica Acta	An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
Journal of Clinical Investigation	Dengue virus-elicited tryptase induces endothelial permeability and shock Pubmed: 31265436
Frontiers in Physiology	Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function Pubmed: 31998139
	Biomarkers of Allergic Asthma and their Association with Serum Parameters
Annals of Tropical Medicine & Public Health	Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
Front Immunol	Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive … 34650557
Microbiol Spectr	Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome Pubmed:35612327

Catalog No.	Related products for research use of Sus scrofa; Porcine (Pig) Organism species	Applications (RESEARCH USE ONLY!)
RPB070Po02	Recombinant Tryptase (TPS)	Positive Control; Immunogen; SDS-PAGE; WB.
RPB070Po01	Recombinant Tryptase (TPS)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB070Po02	Polyclonal Antibody to Tryptase (TPS)	IHC
PAB070Po01	Polyclonal Antibody to Tryptase (TPS)	WB; IHC; ICC; IP.
MAB070Po21	Monoclonal Antibody to Tryptase (TPS)	WB; IHC; ICC; IP.
SEB070Po	ELISA Kit for Tryptase (TPS)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB070Po	Magnetic Luminex Assay Kit for Tryptase (TPS) ,etc.	Magnetic Luminex Assay for Antigen Detection.