Cloud-clone
SKU:APG886Mu01
Active Cold Inducible RNA Binding Protein (CIRBP)
Active Cold Inducible RNA Binding Protein (CIRBP)
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Product No.
APG886Mu01
Organism Species
Mus musculus (Mouse).
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
9.6
Applications
Cell culture; Activity Assays.
UOM
10µg 50µg 200µg 1mg 5mg
ACTIVITY TEST
Cold-inducible RNA-binding protein (CIRBP) plays a critical role in controlling the cellular response upon confronting a variety of cellular stresses, including short wavelength ultraviolet light, hypoxia, and hypothermia. Besides, Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1) has been identified as an interactor of CIRBP, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse CIRBP and recombinant mouse HNRPA1. Briefly, CIRBP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to HNRPA1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CIRBP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and HNRPA1 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of CIRBP with HNRPA1.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| J Cell Mol Med | Normothermic machine perfusion attenuates hepatic ischaemia‐reperfusion injury by inhibiting CIRP‐mediated oxidative stress and mitochondrial fission 34786826 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| APG886Mu01 | Active Cold Inducible RNA Binding Protein (CIRBP) | Cell culture; Activity Assays. |
| RPG886Mu01 | Recombinant Cold Inducible RNA Binding Protein (CIRBP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| SEG886Mu | ELISA Kit for Cold Inducible RNA Binding Protein (CIRBP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
