Recombinant Jagged 1 (JAG1)

Prokaryotic expression

Product No.

RPB807Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Membrane

Predicted Molecular Mass

31.9kDa

Accurate Molecular Mass

32kDa(Analysis of differences refer to the manual)

Residues & Tags

Ser826~Cys1084 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 97%

Isoelectric Point

6.1

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
13	Cross-talk between the Toll-like receptor 4 and Notch1 pathways augments the inflammatory response in the interstitial cells of stenotic human aortic valves PubMed: PMC3506187
Eur J Obstet Gynecol Reprod Biol.	Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosis Pubmed: 24188612
PLoS One.	Augmented Osteogenic Responses in Human Aortic Valve Cells Exposed to oxLDL and TLR4 Agonist: A Mechanistic Role of Notch1 and NF-κB Interaction Pubmed:Pmc4014478
Biochim Biophys Acta.?	Ligation of ICAM-1 on human aortic valve interstitial cells induces the osteogenic response: A critical role of the Notch1-NF-κB pathway in BMP-2 expression Pubmed:25101972
international journal of oral-medical sciences	Jagged1 Stimulates Bone Resorption during Orthodontic Tooth Movement Dn: Jlc
Oncology letters	MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer
Journal of Biological Chemistry	Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328
Cell Death & Disease	Growth hormone induces mitotic catastrophe of glomerular podocytes and contributes to proteinuria 33795655

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPB807Ra01	Recombinant Jagged 1 (JAG1)	Positive Control; Immunogen; SDS-PAGE; WB.