Cloud-clone
SKU:APB090Ra01
Active Glutathione S Transferase Pi (GSTp)
Active Glutathione S Transferase Pi (GSTp)
Couldn't load pickup availability
Product No.
APB090Ra01
Organism Species
Rattus norvegicus (Rat).
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
7.1
Applications
Cell culture; Activity Assays.
UOM
10µg 50µg 200µg 1mg 5mg
ACTIVITY TEST
Glutathione S-transferase P (GSTP1) is a member of Glutathione S-transferases (GSTs) family which play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340nm. The reaction was performed in adding 10μL 200mM glutathione (reduced) and 10μL 100mM CDNB in 980μL 100mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50μL mixed substrates to 50μL recombinant rat GSTP1, mix gentliy, read the absorbance at 340nm for 5min. One unit activity was defined as 1 μmole of CDNB conjugate per minute per 1 mg GSTP1
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| International Journal of Medical Sciences | Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma PubMed: PMC3619117 |
| Mol Neurobiol | Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis Pubmed:27021019 |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering art00007 |
| Neuroscience Bulletin | Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state Pubmed:30019218 |
| Arabian Journal of Chemistry | Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats |
| International Journal of Nature and Life Sciences | The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB090Ra01 | Recombinant Glutathione S Transferase Pi (GSTp) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB090Ra01 | Active Glutathione S Transferase Pi (GSTp) | Cell culture; Activity Assays. |
| PAB090Ra01 | Polyclonal Antibody to Glutathione S Transferase Pi (GSTp) | WB; IHC; ICC; IP. |
| LAB090Ra71 | Biotin-Linked Polyclonal Antibody to Glutathione S Transferase Pi (GSTp) | WB; IHC; ICC. |
| SEB090Ra | ELISA Kit for Glutathione S Transferase Pi (GSTp) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB090Ra | Magnetic Luminex Assay Kit for Glutathione S Transferase Pi (GSTp) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB090Ra01 | ELISA Kit DIY Materials for Glutathione S Transferase Pi (GSTp) | Main materials for "Do It(ELISA Kit) Yourself" |
