Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1)

Prokaryotic expression

Product No.

RPC268Mu01

Organism Species

Mus musculus (Mouse).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Membrane

Predicted Molecular Mass

36.0kDa

Accurate Molecular Mass

34kDa(Analysis of differences refer to the manual)

Residues & Tags

Ala2~Asn292 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

8.6

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Journal of Endocrinology	Uninephrectomy reduces 11β-hydroxysteroid dehydrogenase type 1 and type 2 concomitantly with an increase in blood pressure in rats PubMed: 22739210
Evid Based Complement Alternat Med	Anti-Inflammatory Property of PubMed: 26300946
Molecules	Piper sarmentosum Effects on 11β-Hydroxysteroid Dehydrogenase Type 1 Enzyme in Serumand Bone in Rat Model of Glucocorticoid-Induced Osteoporosis. pubmed:27854305
Bioorganic Chemistry	A novel derivatives of thiazol-4 (5H)-one and their activity in the inhibition of 11β-hydroxysteroid dehydrogenase type 1 Pubmed:29738970
Malaysian Journal of Medical Sciences	An Evidence-Based Review: The Effects of Malaysian Traditional Herbs on Osteoporotic Rat Models
2.583	Application of ELISA Technique and Human Microsomes in the Search for 11β-Hydroxysteroid Dehydrogenase Inhibitors Doi: BioMed Research International
Chemistry & Biodiversity	Synthesis of the N‐methyl derivatives of 2‐aminothiazol‐4 (5H)‐one and their interactions with 11βHSD1‐molecular modeling and in vitro studies Pubmed: 31012543
Oxidative Medicine and Cellular Longevity	Hepatoprotective Effects of Morinda citrifolia Leaf Extract on Ovariectomized Rats Fed with Thermoxidized Palm Oil Diet: Evidence at Histological and Ultrastructural … Pubmed: 31827717

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPC268Mu01	Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1)	Positive Control; Immunogen; SDS-PAGE; WB.
SEC268Mu	ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC268Mu	Magnetic Luminex Assay Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc.	Magnetic Luminex Assay for Antigen Detection.