Recombinant Thrombin/Antithrombin Complex (TAT)

Prokaryotic expression

Product No.

RPA831Hu01

Organism Species

Homo sapiens (Human).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

83.4kDa

Accurate Molecular Mass

83kDa(Analysis of differences refer to the manual)

Residues & Tags

Ile364~Glu622+GGGGS*3+His33~Lys464 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Traits

Freeze-dried powder

Purity

> 80%

Isoelectric Point

7.8

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in ddH₂O to a concentration of 0.1-0.4 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
IUBMB LIFE	Genetic repression of mouse VEGF expression regulates coagulation cascade. PubMed: 21086498
Thrombosis Research	Treatment with unfractionated heparin attenuates coagulation and inflammation in endotoxemic mice ScienceDirect: S0049384811004166
Journal of Thrombosis and Haemostasis	High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality Wiley: source
3	Tissue factor and PAR1 promote microbiota-induced intestinal vascular remodelling Nature: 20120329
Am J Respir Cell Mol Bio	Prolonged Exposure to Sphingosine 1–Phosphate Receptor-1 Agonists Exacerbates Vascular Leak, Fibrosis, and Mortality after Lung Injury PubMed: PMC2993087
PLoS One	Host Defense Peptides of Thrombin Modulate Inflammation and Coagulation in Endotoxin-Mediated Shock and Pseudomonas aeruginosa Sepsis PubMed: PMC3521733
Haemetology	Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India Pubmed: 23992124
Am J Transl Res.	Selected hemostatic parameters in patients with pancreatic tumors. Pubmed:Pmc4297344
PLOS ONE	TFPI1 Mediates Resistance to Doxorubicin in Breast Cancer Cells by Inducing a Hypoxic-Like Response Pubmed:24489651
Leukemia	Novel evidence that crosstalk between the complement, coagulation and fibrinolysis proteolytic cascades is involved in mobilization of hematopoietic stem/progenitor cells (HSPCs) Pubmed:24667943
Anesthesia & Analgesia	Thrombomodulin Improved Liver Injury, Coagulopathy, and Mortality in an Experimental Heatstroke Model in Mice Pubmed:24781566
Cell Transplantation	Evidence of a Pivotal Role for the Distal Part of the Complement Cascade in the Diurnal Release of Hematopoietic Stem Cells Into Peripheral Blood Pubmed:26087465
European Journal of Pharmacology	(S)YS-51, a novel isoquinoline alkaloid, attenuates obesity-associated non-alcoholic fatty liver disease in mice by suppressing lipogenesis, inflammation and coaCavia (Guinea pig )lation Pubmed:27343380
J Thromb Haemost.	Coagulation factor XI vaccination: an alternative strategy to prevent thrombosis. pubmed:27813324
Blood Cells, Inflammation and Infection	Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury pubmed:29202212
SCIENCE TRANSLATIONAL MEDICINE	Platelet-localized FXI promotes a vascular coagulation-inflammatory circuit in arterial hypertension pubmed:28148841
32	Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target pubmed:28289017
Thrombosis andHaemostasis	Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome Pubmed: 29202212
Thrombosis Research	The circadian rhythm of selected parameters of the hemostasis system in healthy people
Experimental Neurobiology	Transduced Tat-aldose Reductase Protects Hippocampal Neuronal Cells against Oxidative Stress-induced Damage Pubmed: 31698553
LIVER TRANSPLANTATION	The impact of dabigatran treatment on sinusoidal protection against hepatic ischemia‐reperfusion injury in mice Pubmed: 33108682
Am J Physiol Heart Circ Physiol	Doxorubicin contributes to thrombus formation and vascular injury via enhancing platelet functions in vivo Pubmed: 32469636
Intensive Care Medicine Experimental	Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental … 34169407
Intensive Care Med Exp	Complement factor D is linked to platelet activation in human and rodent sepsis 34396466

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
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