Recombinant Catechol-O-Methyltransferase (COMT)

Prokaryotic expression

Product No.

RPJ365Hu02

Organism Species

Homo sapiens (Human).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Membrane, Cytoplasm

Predicted Molecular Mass

57.3kDa

Accurate Molecular Mass

57kDa(Analysis of differences refer to the manual)

Residues & Tags

Arg27~Pro271 with N-terminal His and GST Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 80%

Isoelectric Point

5.3

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Reproductive Toxicology	Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary. PubMed: 22750137

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPJ365Hu01	Recombinant Catechol-O-Methyltransferase (COMT)	Positive Control; Immunogen; SDS-PAGE; WB.
APJ365Hu01	Active Catechol-O-Methyltransferase (COMT)	Cell culture; Activity Assays.
RPJ365Hu02	Recombinant Catechol-O-Methyltransferase (COMT)	Positive Control; Immunogen; SDS-PAGE; WB.
PAJ365Hu01	Polyclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB,IHC,ICC/IF
MAJ365Hu21	Monoclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IP.
SEJ365Hu	ELISA Kit for Catechol-O-Methyltransferase (COMT)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ365Hu	Magnetic Luminex Assay Kit for Catechol-O-Methyltransferase (COMT) ,etc.	Magnetic Luminex Assay for Antigen Detection.
KSJ365Hu01	ELISA Kit DIY Materials for Catechol-O-Methyltransferase (COMT)	Main materials for "Do It (ELISA Kit) Yourself".