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SKU:APA297Hu01
Active Ribonuclease A (RNase A)
Active Ribonuclease A (RNase A)
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Product No.
APA297Hu01
Organism Species
Homo sapiens (Human).
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
8.8
Applications
Cell culture; Activity Assays.
UOM
10µg 50µg 200µg 1mg 5mg
ACTIVITY TEST
Ribonuclease A (RNASEA) is a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. RNASEA cleaves RNA on the 3' side of pyrimidine nucleotides. The protein acts to degrade ds-RNA over ss-RNA. The activity of recombinant human RNASEA measured by cleaving yeast RNA. One unit of the enzyme causes an increase in absorbance of 0.001 at 260 nm in 15 min when yeast RNA is hydrolyzed at 50°C and pH 5.0. Pipette 50ulof respective recombinant human RNASEA dilution into 100ul 0.1M sodium acetate buffer, pH 5.0, then add 150ul of 0.15mg/ml yeast RNA. The blank tube use 50ul ultrapure water instead of enzyme dilution. All the all tubes Incubate at 50°C for 15 minutes. Read A260 versus blank.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| International Journal of Molecular Sciences | The Human Host Defense Ribonucleases 1, 3 and 7 Are Elevated in Patients with Sepsis after Major Surgery—A Pilot Study Pubmed:26927088 |
| Investigative Ophthalmology & Visual Science | The Proteomic Landscape in the Vitreous of Patients With Age-Related and Diabetic Retinal Disease Pubmed:30025106 |
| Nat Commun | Human ribonuclease 1 serves as a secretory ligand of ephrin A4 receptor and induces breast tumor initiation 33986289 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APA297Hu01 | Active Ribonuclease A (RNase A) | Cell culture; Activity Assays. |
| RPA297Hu01 | Recombinant Ribonuclease A (RNase A) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA297Hu01 | Polyclonal Antibody to Ribonuclease A (RNase A) | WB; IHC; ICC; IP. |
| LAA297Hu71 | Biotin-Linked Polyclonal Antibody to Ribonuclease A (RNase A) | WB; IHC; ICC. |
| MAA297Hu22 | Monoclonal Antibody to Ribonuclease A (RNase A) | WB; IHC; ICC; IP. |
| SEA297Hu | ELISA Kit for Ribonuclease A (RNase A) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA297Hu | Magnetic Luminex Assay Kit for Ribonuclease A (RNase A) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
