Eukaryotic Fibroblast Growth Factor 21 (FGF21)

Eukaryotic expression

Product No.

EPC918Ra61

Organism Species

Rattus norvegicus (Rat).

Source

Eukaryotic expression

Host

293F cell

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

21.7kDa

Accurate Molecular Mass

28/30kDa(Analysis of differences refer to the manual)

Residues & Tags

Gly25~Ser208 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 95%

Isoelectric Point

4.5

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Lipids in Health and Disease	Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigs Lipidworld: source
Endocrinology	Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression Endocrine:Source
Biochemical and Biophysical Research Communications	Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBH Pubmed:25449271
Free Radic Biol Med	Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathway PubMed: 25843656
Journal of diabetes and its complications	Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. pubmed:27916484
American journal of the medical sciences	Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus pubmed:29078839
Frontiers in Physiology	Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 Pubmed:29593555
	Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим …
Physiol Res	Fibroblast growth factor 21 (FGF21) in children and adolescents with chronic kidney disease. Pubmed: 32469231
Chinese Journal of Osteoporosis	2 型糖尿病患者血清FGF21, 骨连结蛋白和骨转换指标的相关性研究.
Sci Rep	Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats 33833309
Nutrients	The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose
Medicine (Baltimore)	Association of serum fibroblast growth factor 21 with kidney function in a population-based Chinese cohort 34918690
Annals of Agricultural and Environmental Medicine	Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis 34969228
J Magn Reson Imaging	Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074
Lab Invest	Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injury Pubmed:35488034
Molecular Biology Reports	Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377
	High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
APC918Ra01	Active Fibroblast Growth Factor 21 (FGF21)	Cell culture; Activity Assays.
RPC918Ra02	Recombinant Fibroblast Growth Factor 21 (FGF21)	Positive Control; Immunogen; SDS-PAGE; WB.
EPC918Ra61	Eukaryotic Fibroblast Growth Factor 21 (FGF21)	Positive Control; Immunogen; SDS-PAGE; WB.
APC918Ra61	Active Fibroblast Growth Factor 21 (FGF21)	Cell culture; Activity Assays.
RPC918Ra01	Recombinant Fibroblast Growth Factor 21 (FGF21)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC918Ra02	Polyclonal Antibody to Fibroblast Growth Factor 21 (FGF21)	WB; IHC; ICC; IP.
PAC918Ra01	Polyclonal Antibody to Fibroblast Growth Factor 21 (FGF21)	WB; IHC; ICC; IP.
MAC918Ra21	Monoclonal Antibody to Fibroblast Growth Factor 21 (FGF21)	WB; IHC; ICC; IP.
SEC918Ra	ELISA Kit for Fibroblast Growth Factor 21 (FGF21)	Enzyme-linked immunosorbent assay for Antigen Detection.
CEC918Ra	ELISA Kit for Fibroblast Growth Factor 21 (FGF21)	Enzyme-linked immunosorbent assay for Antigen Detection.
WEC918Ra	Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)	Enzyme-linked immunosorbent assay for Antigen Detection.
CCC918Ra	CLIA Kit for Fibroblast Growth Factor 21 (FGF21)	Chemiluminescent immunoassay for Antigen Detection.
LMC918Ra	Magnetic Luminex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc.	Magnetic Luminex Assay for Antigen Detection.
PGC918Ra01	Primer Pair for Fibroblast Growth Factor 21 (FGF21)	PCR