Recombinant Kelch Like ECH Associated Protein 1 (KEAP1)

Prokaryotic expression

Product No.

RPL648Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Nucleus, Cytoplasm

Predicted Molecular Mass

36.0kDa

Accurate Molecular Mass

36kDa(Analysis of differences refer to the manual)

Residues & Tags

Met1~Gln286 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 97%

Isoelectric Point

6.0

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Environmental Toxicology and Pharmacology	Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study pubmed:28192751
Journal of Infection and Chemotherapy	Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicity Pubmed:29753615
Immunopharmacology and immunotoxicology	Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosis Pubmed: 30488751
International Immunopharmacology	S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammation Pubmed: 30665040
Respiratory physiology & neurobiology	Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis Pubmed: 31600583
Vet Sci	Protective and Therapeutic Effects of Orlistat on Metabolic Syndrome and Oxidative Stress in High-Fat Diet-Induced Metabolic Dysfunction-Associated Fatty Liver … 34822647
Antioxidants (Basel)	Hepatoprotective Effect of Bee Bread in Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Rats: Impact on Oxidative Stress and Inflammation 34943134

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPL648Ra01	Recombinant Kelch Like ECH Associated Protein 1 (KEAP1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAL648Ra01	Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1)	WB; IHC; ICC; IP.