Eukaryotic Epidermal Growth Factor (EGF)

Eukaryotic expression

Product No.

EPA560Po61

Organism Species

Sus scrofa; Porcine (Pig).

Source

Eukaryotic expression

Host

293F cell

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Membrane

Predicted Molecular Mass

8.7kDa

Accurate Molecular Mass

17-20kDa(Analysis of differences refer to the manual)

Residues & Tags

Asn970~Arg1022 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

5.5

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

5X10^5

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine	Citations
Journal of Pediatric Surgery	The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model PubMed: 20438940
Biomark Insights	Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism PubMed: PMC3623607
Plos one	The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor PubMed: PMC3590171
Diabetes.	Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity Pubmed:25795216
Eur Rev Med Pharmacol Sci.?	Association of colon adenomas and skin tags: coincidence or coexistence? Pubmed:24763889
Int J Clin Exp Med.	The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972
ACS Applied Materials & Interfaces	Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness. pubmed:27709887
Canadian Journal of Physiology and Pharmacology	The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats pubmed:28177680
Journal of Controlled Release	A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury pubmed:28522195
Scientific Reports	Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets pubmed:28811665
International Journal of Nanomedicine	Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human … Pubmed: 30233172
Neoplasia	Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine Pubmed: 33212364
Aesthet Surg J	Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water 33428732
Cell Death & Disease	Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization 34006822
Stem Cells International	The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡­ 33381190
Molecular Biology Reports	Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377
Biomaterials Advances	Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats
Authorea	Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy
Biointerface Research in Applied Chemistry	Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats

Catalog No.	Related products for research use of Sus scrofa; Porcine (Pig) Organism species	Applications (RESEARCH USE ONLY!)
APA560Po61	Active Epidermal Growth Factor (EGF)	Cell culture; Activity Assays.
RPA560Po01	Recombinant Epidermal Growth Factor (EGF)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA560Po02	Recombinant Epidermal Growth Factor (EGF)	Positive Control; Immunogen; SDS-PAGE; WB.
EPA560Po61	Eukaryotic Epidermal Growth Factor (EGF)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA560Po02	Polyclonal Antibody to Epidermal Growth Factor (EGF)	WB; IHC; ICC; IP.
PAA560Po01	Polyclonal Antibody to Epidermal Growth Factor (EGF)	WB; IHC; ICC; IP.
LAA560Po81	FITC-Linked Polyclonal Antibody to Epidermal Growth Factor (EGF)	WB; IHC; ICC; IF.
LAA560Po71	Biotin-Linked Polyclonal Antibody to Epidermal Growth Factor (EGF)	WB; IHC; ICC.
MAA560Po21	Monoclonal Antibody to Epidermal Growth Factor (EGF)	WB; IHC; ICC; IP.
SEA560Po	ELISA Kit for Epidermal Growth Factor (EGF)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA560Po	Magnetic Luminex Assay Kit for Epidermal Growth Factor (EGF) ,etc.	Magnetic Luminex Assay for Antigen Detection.