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LMA701Ge

Magnetic Luminex Assay Kit for 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) ,etc.

Magnetic Luminex Assay Kit for 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) ,etc.

$955.00 USD
$955.00 USD
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Detection range
Size
Sensitivity

Product No.

LMA701Ge

Organism Species

Pan-species (General).

Sample Type

Serum, plasma and other biological fluids

Test Method

Competitive Inhibition

Assay Length

1.5h

Detection Range

1.95-2000pg/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 0.65 pg/mL.

UOM

1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex

 

Specificity

This assay has high sensitivity and excellent specificity for detection of 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) ,etc..
No significant cross-reactivity or interference between 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) ,etc. and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) ,etc. in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-99 94
EDTA plasma(n=5) 80-94 90
heparin plasma(n=5) 78-95 89
sodium citrate plasma(n=5) 79-88 81

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-96% 88-104% 94-102% 91-98%
EDTA plasma(n=5) 97-105% 95-105% 96-103% 97-105%
heparin plasma(n=5) 94-105% 96-103% 78-94% 88-95%
sodium citrate plasma(n=5) 97-105% 82-101% 78-94% 80-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine. 

     

    Magazine Citations
    International Journal of Clinical and Experimental Medicine Relationships between glucose fluctuations and oxidative stress in short duration type 2 diabetes treated with sensor-augmented insulin pump. ISSN:1940-5901
    BMC Veterinary Research Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome Pubmed: 30404636
    Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
    CEA701Ge ELISA Kit for 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) Enzyme-linked immunosorbent assay for Antigen Detection.
    LMA701Ge Magnetic Luminex Assay Kit for 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a, 8-iso-PGF2alpha, 8-iso-PGF2a) ,etc. Magnetic Luminex Assay for Antigen Detection.
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