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SKU:LMG031Ge
Magnetic Luminex Assay Kit for Sphingosine-1-Phosphate (S1P) ,etc.
Magnetic Luminex Assay Kit for Sphingosine-1-Phosphate (S1P) ,etc.
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Product No.
LMG031Ge
Organism Species
Pan-species (General).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.98-1000ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.327 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Sphingosine-1-Phosphate (S1P) ,etc..
No significant cross-reactivity or interference between Sphingosine-1-Phosphate (S1P) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Sphingosine-1-Phosphate (S1P) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Sphingosine-1-Phosphate (S1P) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-101 | 84 |
| EDTA plasma(n=5) | 82-101 | 90 |
| heparin plasma(n=5) | 98-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine-1-Phosphate (S1P) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine-1-Phosphate (S1P) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sphingosine-1-Phosphate (S1P) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-97% | 91-103% | 87-96% | 97-105% |
| EDTA plasma(n=5) | 99-105% | 98-105% | 80-97% | 97-105% |
| heparin plasma(n=5) | 81-88% | 88-96% | 94-101% | 79-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:S1P) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| International Journal of Biochemistry & Cell Biology | Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signaling Pubmed:29940125 |
| Cell Reports | Nppb Neurons Are Sensors of Mast Cell-Induced Itch Pubmed: 30917312 |
| Clinical and Experimental Pharmacology and Physiology | SPNS 2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway Pubmed: 31206801 |
| Sci Rep | Role of miR-506 in ulcerative colitis associated with primary sclerosing cholangitis 33980925 |
| Can J Physiol Pharmacol | The S1PR1 Agonist SEW2871 promotes the Survival of Skin Flap 34310896 |
| Andrology | Berberine ameliorates erectile dysfunction in rats with streptozotocin‐induced diabetes mellitus through the attenuation of apoptosis by inhibiting the SPHK1/S1P … 34674380 |
| EBioMedicine | α-Ketoglutarate Inhibits Thrombosis and Inflammation by Prolyl Hydroxylase-2 Mediated Inactivation of Phospho-Akt 34740102 |
| Biomedicines | Implication of Sphingolipid Metabolism Gene Dysregulation and Cardiac Sphingosine-1-Phosphate Accumulation in Heart Failure Pubmed:35052814 |
| Sci Rep | Sphk2 deletion is involved in structural abnormalities and Th17 response but does not aggravate colon inflammation induced by sub-chronic stress Pubmed:35260749 |
| Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS | |
| Network Pharmacology Study on the Diuretic Effect of Plantaginis Semen and its Anti-Hypertensive Mechanism |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CEG031Ge | ELISA Kit for Sphingosine-1-Phosphate (S1P) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMG031Ge | Magnetic Luminex Assay Kit for Sphingosine-1-Phosphate (S1P) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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