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SKU:LMC026Mu
Magnetic Luminex Assay Kit for Salusin Beta (SALb) ,etc.
Magnetic Luminex Assay Kit for Salusin Beta (SALb) ,etc.
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Product No.
LMC026Mu
Organism Species
Mus musculus (Mouse).
Sample Type
serum, plasma and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.39-400pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.13 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Salusin Beta (SALb) ,etc..
No significant cross-reactivity or interference between Salusin Beta (SALb) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Salusin Beta (SALb) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Salusin Beta (SALb) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-101 | 96 |
| EDTA plasma(n=5) | 85-99 | 93 |
| heparin plasma(n=5) | 82-101 | 85 |
| sodium citrate plasma(n=5) | 92-101 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Salusin Beta (SALb) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Salusin Beta (SALb) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Salusin Beta (SALb) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 87-104% | 78-98% | 88-103% |
| EDTA plasma(n=5) | 91-102% | 99-105% | 78-94% | 97-105% |
| heparin plasma(n=5) | 83-101% | 78-104% | 95-102% | 80-104% |
| sodium citrate plasma(n=5) | 78-97% | 90-97% | 90-101% | 80-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:SALb) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Journal of Maternal-Fetal and Neonatal Medicine | Serum levels of apelin, salusin-alpha, and salusin-beta in normal pregnancy and preeclampsia Informa: 14767058.2012.660221 |
| Peptides | The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass PubMed: 22884920 |
| Department of Medical Biochemistry and Clinical Biochemistry | Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows ScienceDirect: S0196978113000533 |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses. PubMed: 23178004 |
| Biotech Histochem. | Serum concentration and kidney expression of salusin-α and salusin-β in rats with metabolic syndrome induced by fructose. PubMed: 23244237 |
| Gynecological Endocrinology, | Salusins, newly identified regulators of hemodynamics and mitogenesis, increase in polycystic ovarian syndrome. PubMed: 22827297 |
| Peptides | Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus ScienceDirect: S0196978113003100 |
| Biotechnic & Histochemistry | Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome Com:Source |
| Med Sci Monit. | Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome Pubmed:Pmc4136931 |
| Acta Physiologica | Superoxide anions involved in sympathoexcitation and pressor effects of salusin-β in paraventricular nucleus in hypertensive rats Pubmed:24304512 |
| Pediatr Nephrol. | Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population? Pubmed:25245503 |
| European Journal of Dermatology | Serum salusin-α and salusin-β levels in patients with psoriasis PubMed: 26055619 |
| J Investig Med | Serum Salusin-β Levels Are Associated With the Presence and Severity of Coronary Artery Disease PubMed: 25730454 |
| Genet Test Mol Biomarkers. | Serum Salusin-β Levels Are Correlated with Slow Coronary Flow. Pubmed:27227272 |
| Sci Rep. | Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. pubmed:28230187 |
| Cell Death Dis. | Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy pubmed:28333148 |
| Oxidative Medicine and Cellular Longevity | Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma 10.1155:2017 |
| International Journal Of Cardiology | Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus Pubmed: 30581101 |
| Multiple Sclerosis and Related Disorders | Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis Pubmed: 30738876 |
| cardiology in the young | Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity Pubmed: 31486354 |
| Redox Biology | Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathway Pubmed: 31884071 |
| Journal of international medical research | Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis Pubmed: 32054363 |
| cardiovascular drugs and therapy | A TOR2A Gene Product: Salusin-β Contributes to Attenuated Vasodilatation of Spontaneously Hypertensive Rats. Pubmed: 32458319 |
| Serum level of salusin β as an indicator of metabolic disorders in Acute Lymphoblastic Leucaemia and Wilms Tumour survivors | |
| Mol Med Rep | Salusin‑β participates in high glucose‑induced HK‑2 cell ferroptosis in a Nrf‑2‑dependent manner 34296310 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| CPC026Mu21 | OVA Conjugated Salusin Beta (SALb) | Immunogen; SDS-PAGE; WB. |
| CEC026Mu | ELISA Kit for Salusin Beta (SALb) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC026Mu | Magnetic Luminex Assay Kit for Salusin Beta (SALb) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSC026Mu11 | ELISA Kit DIY Materials for Salusin Beta (SALb) | Main materials for "Do It (ELISA Kit) Yourself". |
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