Cloud-clone
SKU:LME038Ra
Magnetic Luminex Assay Kit for Neurofilament, Light Polypeptide (NEFL) ,etc.
Magnetic Luminex Assay Kit for Neurofilament, Light Polypeptide (NEFL) ,etc.
Couldn't load pickup availability
Product No.
LME038Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
9.77-10000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 3.257 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL) ,etc..
No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Neurofilament, Light Polypeptide (NEFL) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Neurofilament, Light Polypeptide (NEFL) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-97 | 87 |
| EDTA plasma(n=5) | 93-101 | 97 |
| heparin plasma(n=5) | 91-99 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurofilament, Light Polypeptide (NEFL) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurofilament, Light Polypeptide (NEFL) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neurofilament, Light Polypeptide (NEFL) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-101% | 93-105% | 80-95% | 80-93% |
| EDTA plasma(n=5) | 98-105% | 81-91% | 79-93% | 78-91% |
| heparin plasma(n=5) | 78-98% | 90-97% | 92-105% | 88-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:NEFL) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Acta Neurologica Scandinavica | Effect of carotid endarterectomy on brain damage markers Pubmed:27126899 |
| Acta Neurologica Scandinavica | Effect of carotid endarterectomy on brain damage markers. pubmed:27126899 |
| journal of neuroimmunology | Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implications Pubmed: 31254929 |
| Cerebellum | Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia 33893614 |
| Medicine (Baltimore) | Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis 34678870 |
| J Clin Med | Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients … Pubmed:35566558 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| CEE038Ra | ELISA Kit for Neurofilament, Light Polypeptide (NEFL) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME038Ra | Magnetic Luminex Assay Kit for Neurofilament, Light Polypeptide (NEFL) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
Share
