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SKU:LMB480Ga
Magnetic Luminex Assay Kit for Mannose Binding Lectin (MBL) ,etc.
Magnetic Luminex Assay Kit for Mannose Binding Lectin (MBL) ,etc.
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Product No.
LMB480Ga
Organism Species
Chicken (Gallus).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.05-50ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.017 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL) ,etc..
No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Mannose Binding Lectin (MBL) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Mannose Binding Lectin (MBL) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-96 | 92 |
| EDTA plasma(n=5) | 81-99 | 90 |
| heparin plasma(n=5) | 82-99 | 95 |
| sodium citrate plasma(n=5) | 78-99 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mannose Binding Lectin (MBL) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-89% | 94-101% | 88-102% | 96-104% |
| EDTA plasma(n=5) | 79-104% | 96-104% | 84-94% | 81-103% |
| heparin plasma(n=5) | 80-101% | 89-96% | 80-91% | 82-90% |
| sodium citrate plasma(n=5) | 78-89% | 83-103% | 89-97% | 92-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:MBL) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| American Journal of Nephrology | Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. Karger: 000255439 |
| Current Eye Research | Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle Glaucoma Ingenta: art00007 |
| Journal of Clinical Immunology | Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. PubMed: 22941511 |
| Clinical and Experimental Immunology | Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathy PubMed: PMC3406374 |
| Clin J Am Soc Nephrol. | Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GN Pubmed: 24115193 |
| ACTA TROPICA | Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis Pubmed: 32827456 |
| PLoS One | Complement activation profile of patients with primary focal segmental glomerulosclerosis Pubmed: 32569286 |
| LIFE SCIENCES | The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes 34242658 |
| Catalog No. | Related products for research use of Chicken (Gallus) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB480Ga01 | Recombinant Mannose Binding Lectin (MBL) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB480Ga01 | Polyclonal Antibody to Mannose Binding Lectin (MBL) | WB; IHC; ICC; IP. |
| MAB480Ga21 | Monoclonal Antibody to Mannose Binding Lectin (MBL) | WB; IHC; ICC; IP. |
| SEB480Ga | ELISA Kit for Mannose Binding Lectin (MBL) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB480Ga | Magnetic Luminex Assay Kit for Mannose Binding Lectin (MBL) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB480Ga01 | ELISA Kit DIY Materials for Mannose Binding Lectin (MBL) | Main materials for "Do It (ELISA Kit) Yourself". |
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