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Cloud-clone

SKU:LMA430Ra

Magnetic Luminex Assay Kit for Sirtuin 2 (SIRT2) ,etc.

Magnetic Luminex Assay Kit for Sirtuin 2 (SIRT2) ,etc.

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Detection range
Size
Sensitivity

Product No.

LMA430Ra

Organism Species

Rattus norvegicus (Rat).

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Test Method

Double-antibody Sandwich

Assay Length

3.5h

Detection Range

0.1-100ng/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 0.033 ng/mL.

UOM

1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex

 

1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

 

Magazine Citations
Brain Research Bulletin The effects of melatonin and curcumin on the expression of SIRT2, Bcl-2 and Bax in the hippocampus of adult rats Pubmed:29325994
Сибирский федеральный университет Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте
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