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SKU:LMC704Mu
Magnetic Luminex Assay Kit for Oxoguanine Glycosylase 1 (OGG1) ,etc.
Magnetic Luminex Assay Kit for Oxoguanine Glycosylase 1 (OGG1) ,etc.
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Product No.
LMC704Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
1.95-2000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.65 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Oxoguanine Glycosylase 1 (OGG1) ,etc..
No significant cross-reactivity or interference between Oxoguanine Glycosylase 1 (OGG1) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Oxoguanine Glycosylase 1 (OGG1) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Oxoguanine Glycosylase 1 (OGG1) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-102 | 90 |
| EDTA plasma(n=5) | 80-101 | 86 |
| heparin plasma(n=5) | 86-96 | 93 |
| sodium citrate plasma(n=5) | 84-91 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxoguanine Glycosylase 1 (OGG1) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxoguanine Glycosylase 1 (OGG1) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxoguanine Glycosylase 1 (OGG1) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-98% | 85-96% | 79-92% | 86-99% |
| EDTA plasma(n=5) | 86-98% | 78-94% | 89-99% | 78-101% |
| heparin plasma(n=5) | 81-97% | 92-101% | 79-88% | 80-105% |
| sodium citrate plasma(n=5) | 94-101% | 80-99% | 97-104% | 87-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:OGG1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Journal of the Neurological Sciences | The levels of 7,8-dihydrodeoxyguanosine (8-oxoG) and 8-oxoguanine DNAglycosylase 1 (OGG1) - A potential diagnostic biomarkers of Alzheimer'sdisease. pubmed:27538622 |
| Indian Journal of Animal Research | Expression Pattern of MALAT-1 Gene and Oxidative DNA Damage in Nigeria Men with Prostate Carcinoma. |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC704Mu01 | Recombinant Oxoguanine Glycosylase 1 (OGG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC704Mu02 | Recombinant Oxoguanine Glycosylase 1 (OGG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC704Mu01 | Polyclonal Antibody to Oxoguanine Glycosylase 1 (OGG1) | WB; IHC; ICC; IP. |
| PAC704Mu02 | Polyclonal Antibody to Oxoguanine Glycosylase 1 (OGG1) | WB; IHC; ICC; IP. |
| SEC704Mu | ELISA Kit for Oxoguanine Glycosylase 1 (OGG1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC704Mu | Magnetic Luminex Assay Kit for Oxoguanine Glycosylase 1 (OGG1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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