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Magnetic Luminex Assay Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc.
Magnetic Luminex Assay Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc.
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Product No.
LMA639Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, urine and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.2-200ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.067 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc..
No significant cross-reactivity or interference between Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-101 | 97 |
| EDTA plasma(n=5) | 80-102 | 95 |
| heparin plasma(n=5) | 95-103 | 99 |
| sodium citrate plasma(n=5) | 79-98 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-98% | 85-96% | 79-92% | 86-99% |
| EDTA plasma(n=5) | 78-91% | 94-102% | 89-101% | 87-105% |
| heparin plasma(n=5) | 87-101% | 93-101% | 84-99% | 86-93% |
| sodium citrate plasma(n=5) | 83-101% | 80-91% | 78-103% | 78-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:NTXI) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Nutrition | Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacity ScienceDirect: S0899900709004717 |
| Bone | Differential mRNA expression profiles in proximal tibia of aged rats in response to ovariectomy and low-Ca diet ScienceDirect: S875632820800776X |
| Journal of Orthopaedic Research | Promotion of bone formation by naringin in a titanium particle‐induced diabetic murine calvarial osteolysis model Wiley: Source |
| The Journal of Clinical Endocrinology & Metabolism | A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular Risk PubMed: PMC3462931 |
| Acta Paediatr.? | Biochemical markers of bone resorption are present in human milk: implications for maternal and neonatal bone metabolism Pubmed:25109232 |
| PLoS One. | An NMR Metabolomic Study on the Effect of Alendronate in Ovariectomized Mice Pubmed:25184758 |
| J Bone Miner Res | Strontium Ranelate Reduces the Fracture Incidence in a Growing Mouse Model of Osteogenesis Imperfecta PubMed: 26679066 |
| Journal of Functional Foods | Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561 |
| Archives of Oral Biology | Impact of a chronic smoking habit on the osteo-immunoinflammatory mediators in the peri-implant fluid of clinically healthy dental implants. pubmed:27328151 |
| Journal of Periodontology | Impact of a triclosan‐containing toothpaste during the progression of experimental peri‐implant mucositis: Clinical parameters and local pattern of osteo … Pubmed:29520826 |
| Clinical oral implants research | Triclosan‐containing fluoride toothpaste on clinical parameters and osteo‐inflammatory mediators when applied in a stent during experimental peri‐implant mucositis … Pubmed: 30659666 |
| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities Pubmed: 31739209 |
| Bioengineering (Basel) | Material-Dependent Formation and Degradation of Bone Matrix—Comparison of Two Cryogels Pubmed: 32517006 |
| BioMed Research International | Comparable Effects of Strontium Ranelate and Alendronate Treatment on Fracture Reduction in a Mouse Model of Osteogenesis Imperfecta 33506016 |
| PHARMACEUTICAL BIOLOGY | Effects of icariin on the fracture healing in young and old rats and its mechanism 34511043 |
| Evid Based Complement Alternat Med | Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway 34512780 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| MEA639Mu | Mini Samples ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA639Mu | ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA639Mu | Magnetic Luminex Assay Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSA639Mu11 | ELISA Kit DIY Materials for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) | Main materials for "Do It (ELISA Kit) Yourself". |
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