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SKU:LMC110Ra
Magnetic Luminex Assay Kit for Growth Differentiation Factor 5 (GDF5) ,etc.
Magnetic Luminex Assay Kit for Growth Differentiation Factor 5 (GDF5) ,etc.
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Product No.
LMC110Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.01-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.003 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Growth Differentiation Factor 5 (GDF5) ,etc..
No significant cross-reactivity or interference between Growth Differentiation Factor 5 (GDF5) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Growth Differentiation Factor 5 (GDF5) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Differentiation Factor 5 (GDF5) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-101 | 95 |
| EDTA plasma(n=5) | 89-97 | 94 |
| heparin plasma(n=5) | 78-96 | 89 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 5 (GDF5) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 5 (GDF5) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Differentiation Factor 5 (GDF5) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-93% | 97-105% | 85-96% | 96-105% |
| EDTA plasma(n=5) | 89-102% | 93-105% | 90-98% | 96-105% |
| heparin plasma(n=5) | 83-91% | 93-101% | 91-98% | 92-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:GDF5) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Carbohydrate Polymers | ZrO2 surface chemically coated with hyaluronic acid hydrogel loading GDF-5 for osteogenesis in dentistry PubMed: 23218279 |
| Bone | Photo-cured hyaluronic acid-based hydrogels containing growth and differentiation factor 5 (GDF-5) for bone tissue regeneration ScienceDirect: S875632821300481X |
| J Nanosci Nanotechnol | In Vitro Osteogenic Differentiation Enhanced by Zirconia Coated with Nano-Layered Growth and Differentiation Factor-5 Pubmed:27398455 |
| Macromolecular Research | Osteoblastic Differentiation of Functionalized Biphasic Hydroxyapatite and b-Tricalcium Phosphate with Recombinant Human Growth and Differentiation (rhGDF-5) |
| Pharmaceutics | 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells 34575523 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC110Ra01 | Recombinant Growth Differentiation Factor 5 (GDF5) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC110Ra01 | Polyclonal Antibody to Growth Differentiation Factor 5 (GDF5) | WB; IHC; ICC; IP. |
| MAC110Ra21 | Monoclonal Antibody to Growth Differentiation Factor 5 (GDF5) | WB; IHC; ICC; IP. |
| SEC110Ra | ELISA Kit for Growth Differentiation Factor 5 (GDF5) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCC110Ra | CLIA Kit for Growth Differentiation Factor 5 (GDF5) | Chemiluminescent immunoassay for Antigen Detection. |
| LMC110Ra | Magnetic Luminex Assay Kit for Growth Differentiation Factor 5 (GDF5) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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