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SKU:LMC869Mu
Magnetic Luminex Assay Kit for Serglycin (SRGN) ,etc.
Magnetic Luminex Assay Kit for Serglycin (SRGN) ,etc.
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Product No.
LMC869Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
2.93-3000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.977 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Serglycin (SRGN) ,etc..
No significant cross-reactivity or interference between Serglycin (SRGN) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Serglycin (SRGN) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Serglycin (SRGN) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-105 | 101 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 99-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serglycin (SRGN) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serglycin (SRGN) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Serglycin (SRGN) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-101% | 78-94% | 91-101% | 79-101% |
| EDTA plasma(n=5) | 83-94% | 94-102% | 95-103% | 84-103% |
| heparin plasma(n=5) | 98-105% | 86-103% | 99-105% | 80-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:SRGN) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Irish Journal of Medical Science | The role of increased synovial fluid A disintegrin and metalloproteinase with thrombospondin motifs4 and serglycin levels in osteoarthritis Pubmed: 30536194 |
| MOLECULAR AND CELLULAR BIOCHEMISTRY | SRGN, a new identified shear-stress-responsive gene in endothelial cells Pubmed: 32712749 |
| Theranostics | Significance of serglycin and its binding partners in autocrine promotion of metastasis in esophageal cancer 33456569 |
| CELLULAR ONCOLOGY | Cancer©\associated fibroblasts secrete hypoxia©\induced serglycin to promote head and neck squamous cell carcinoma tumor cell growth in vitro and in vivo by ¡ 33651283 |
| Cell Death Dis | Serglycin induces osteoclastogenesis and promotes tumor growth in giant cell tumor of bone 34556636 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC869Mu01 | Recombinant Serglycin (SRGN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC869Mu01 | Polyclonal Antibody to Serglycin (SRGN) | WB; IHC; ICC; IP. |
| LAC869Mu81 | FITC-Linked Polyclonal Antibody to Serglycin (SRGN) | WB; IHC; ICC; IF. |
| LAC869Mu71 | Biotin-Linked Polyclonal Antibody to Serglycin (SRGN) | WB; IHC; ICC. |
| SEC869Mu | ELISA Kit for Serglycin (SRGN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC869Mu | Magnetic Luminex Assay Kit for Serglycin (SRGN) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSC869Mu01 | ELISA Kit DIY Materials for Serglycin (SRGN) | Main materials for "Do It (ELISA Kit) Yourself". |
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