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SKU:LMB903Mu
Magnetic Luminex Assay Kit for Ficolin 3 (FCN3) ,etc.
Magnetic Luminex Assay Kit for Ficolin 3 (FCN3) ,etc.
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Product No.
LMB903Mu
Organism Species
Mus musculus (Mouse).
Sample Type
serum, plasma and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.98-1000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.327 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Ficolin 3 (FCN3) ,etc..
No significant cross-reactivity or interference between Ficolin 3 (FCN3) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Ficolin 3 (FCN3) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Ficolin 3 (FCN3) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-102 | 99 |
| EDTA plasma(n=5) | 83-98 | 94 |
| heparin plasma(n=5) | 86-97 | 90 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ficolin 3 (FCN3) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ficolin 3 (FCN3) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ficolin 3 (FCN3) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 83-99% | 79-104% | 94-102% |
| EDTA plasma(n=5) | 79-89% | 83-94% | 96-105% | 81-105% |
| heparin plasma(n=5) | 80-101% | 87-103% | 97-105% | 82-91% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:FCN3) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| American Journal of Ophthalmology | Correlation between Ficolin-3 and Vascular Endothelial Growth Factor-to-Pigment Epithelium-Derived Factor Ratio in the Vitreous of Eyes with Proliferative Diabetic Retinopathy ScienceDirect: S0002939411004491 |
| Liver International | Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 5-D DIGE Pubmed: 23944848 |
| Fundamental & Clinical Pharmacology | The determination of histopathological and biochemical effects of the rabbit knee joint injected dexketoprofen trometamol Onlinelibrary: fcp.12074 |
| The Annals of Thoracic Surgery | Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases Pubmed: 24565402 |
| Journal of Proteome Research | Targeted proteomics predict a sustained complete-response after transarterial chemoembolization and clinical outcomes in patients with hepatocellular carcinoma: a prospective cohort study pubmed:28112944 |
| BMC Cancer | Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis Pubmed:29386009 |
| ACTA TROPICA | Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis Pubmed: 32827456 |
| Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848 |
| Immunol Lett | Ficolin-3 in Rheumatic Fever and Rheumatic Heart Disease 33232720 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| SEB903Mu | ELISA Kit for Ficolin 3 (FCN3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB903Mu | Magnetic Luminex Assay Kit for Ficolin 3 (FCN3) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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