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SKU:LMB022Ge
Magnetic Luminex Assay Kit for Beta-Hydroxybutyric Acid (bHB) ,etc.
Magnetic Luminex Assay Kit for Beta-Hydroxybutyric Acid (bHB) ,etc.
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Product No.
LMB022Ge
Organism Species
Pan-species (General).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.1-100ug/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.033 ug/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Beta-Hydroxybutyric Acid (bHB) ,etc..
No significant cross-reactivity or interference between Beta-Hydroxybutyric Acid (bHB) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Beta-Hydroxybutyric Acid (bHB) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-Hydroxybutyric Acid (bHB) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 89-103 | 99 |
| EDTA plasma(n=5) | 94-105 | 101 |
| heparin plasma(n=5) | 89-97 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Hydroxybutyric Acid (bHB) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Hydroxybutyric Acid (bHB) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-Hydroxybutyric Acid (bHB) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-105% | 89-103% | 97-104% | 92-101% |
| EDTA plasma(n=5) | 89-101% | 98-105% | 78-89% | 98-105% |
| heparin plasma(n=5) | 92-101% | 80-102% | 83-103% | 92-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:bHB) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Endocrinology | Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression Endocrine:Source |
| Chem Commun (Camb) | A bioelectronic system for insulin release triggered by ketone body mimicking diabetic ketoacidosis in vitro PubMed: 25846235 |
| PLoS One | Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine pubmed:27501393 |
| Buffalo Bulletin | Alterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes |
| Journal of Integrative Agriculture | Temporal dynamics of nutrient balance, plasma biochemical and immune traits, and liver function in transition dairy cows |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CPB022Ge11 | BSA Conjugated Beta-Hydroxybutyric Acid (bHB) | Immunogen; SDS-PAGE; WB. |
| CPB022Ge21 | OVA Conjugated Beta-Hydroxybutyric Acid (bHB) | Immunogen; SDS-PAGE; WB. |
| PAB022Ge01 | Polyclonal Antibody to Beta-Hydroxybutyric Acid (bHB) | WB; IHC; ICC; IP. |
| CEB022Ge | ELISA Kit for Beta-Hydroxybutyric Acid (bHB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB022Ge | Magnetic Luminex Assay Kit for Beta-Hydroxybutyric Acid (bHB) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB022Ge11 | ELISA Kit DIY Materials for Beta-Hydroxybutyric Acid (bHB) | Main materials for "Do It (ELISA Kit) Yourself". |
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