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SKU:LMD295Ra
Magnetic Luminex Assay Kit for Cytochrome P450 1A1 (CYP1A1) ,etc.
Magnetic Luminex Assay Kit for Cytochrome P450 1A1 (CYP1A1) ,etc.
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Product No.
LMD295Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.02-20ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.007 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex.
Specificity
This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 1A1 (CYP1A1) ,etc..
No significant cross-reactivity or interference between Cytochrome P450 1A1 (CYP1A1) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cytochrome P450 1A1 (CYP1A1) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Cytochrome P450 1A1 (CYP1A1) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-104 | 99 |
| EDTA plasma(n=5) | 80-89 | 84 |
| heparin plasma(n=5) | 92-101 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 1A1 (CYP1A1) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 1A1 (CYP1A1) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytochrome P450 1A1 (CYP1A1) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-103% | 91-104% | 87-99% | 92-105% |
| EDTA plasma(n=5) | 78-99% | 95-105% | 84-92% | 87-104% |
| heparin plasma(n=5) | 91-99% | 92-101% | 95-102% | 94-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:CYP1A1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats Pubmed:25733458 |
| The Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,3 PubMed: 25733458 |
| Molecular Nutrition Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats Pubmed:27133590 |
| International Journal of Chemical and Natural Science | Caspase-3, Bcl-2, p53, CYP1A1 and COX -2 as a potential target in chemoprevention of Benzo (a) pyrene-induced lung carcinogenesis in mice: Role of thymoquinone portal:uploads |
| International Journal of Pharma Sciences | Chemopreventive Role of Curcumin in benzo(A)pyrene induced Lung Carcinogenesis in mice via-modulation of Bcl-2, p53, Caspase-3, Cyp1A1, COX-2 and antioxidant defense system in Lung tissues portal:uploads |
| Molecular Nutrition & Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. pubmed:27133590 |
| Biochemistry (Mosc) | Cytochrome P450 1A1 (CYP1A1) Catalyzes Lipid Peroxidation of Oleic Acid-Induced HepG2 Cells Pubmed:29738693 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD295Ra01 | Recombinant Cytochrome P450 1A1 (CYP1A1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD295Ra01 | Active Cytochrome P450 1A1 (CYP1A1) | Cell culture; Activity Assays. |
| PAD295Ra01 | Polyclonal Antibody to Cytochrome P450 1A1 (CYP1A1) | WB; IHC; ICC; IP. |
| MAD295Ra21 | Monoclonal Antibody to Cytochrome P450 1A1 (CYP1A1) | WB; IHC; ICC; IP. |
| SED295Ra | ELISA Kit for Cytochrome P450 1A1 (CYP1A1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD295Ra | Magnetic Luminex Assay Kit for Cytochrome P450 1A1 (CYP1A1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSD295Ra01 | ELISA Kit DIY Materials for Cytochrome P450 1A1 (CYP1A1) | Main materials for "Do It (ELISA Kit) Yourself". |
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