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SKU:LMD317Ra
Magnetic Luminex Assay Kit for Glycogen Synthase Kinase 3 Beta (GSK3b) ,etc.
Magnetic Luminex Assay Kit for Glycogen Synthase Kinase 3 Beta (GSK3b) ,etc.
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Product No.
LMD317Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
tissue homogenates, cell lysates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.05-50ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.017 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex.
Specificity
This assay has high sensitivity and excellent specificity for detection of Glycogen Synthase Kinase 3 Beta (GSK3b) ,etc..
No significant cross-reactivity or interference between Glycogen Synthase Kinase 3 Beta (GSK3b) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Glycogen Synthase Kinase 3 Beta (GSK3b) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Glycogen Synthase Kinase 3 Beta (GSK3b) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-102 | 98 |
| EDTA plasma(n=5) | 89-103 | 96 |
| heparin plasma(n=5) | 92-104 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycogen Synthase Kinase 3 Beta (GSK3b) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycogen Synthase Kinase 3 Beta (GSK3b) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glycogen Synthase Kinase 3 Beta (GSK3b) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-93% | 92-101% | 95-104% | 79-93% |
| EDTA plasma(n=5) | 90-98% | 94-101% | 93-101% | 99-105% |
| heparin plasma(n=5) | 84-94% | 94-102% | 85-97% | 90-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:GSK3b) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Neurological Research | Sorafenib and lithium chloride combination treatment shows promising synergistic effects in human glioblastoma multiforme cells in vitro but midkine is not implicated Maneyonline:Source |
| Int J Clin Exp Med | Glycogen synthase kinase-3β (GSK-3β) rs334558 polymorphism is not associated with mild cognitive impairment in Chinese Han type 2 diabetic patients ijcem0038535.pdf |
| Bulletin of Experimental Biology and Medicine | Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia Pubmed:29308565 |
| Biomedicine & Pharmacotherapy | Neuroprotective effects of Bergenia ciliata on NMDA induced injury in SH-SY5Y cells and attenuation of cognitive deficits in scopolamine induced amnesia in rats Pubmed: 30227331 |
| FEATURES OF DISTRIBUTION OF SOYBEAN AGGLUTININ (SBA) RECEPTORS IN THE EXTRACELLULAR MATRIX OF THE MENISCI OF RAT KNEE JOINT … | |
| Br J Haematol | Glycogen synthase kinase 3β/CCR6‐positive bone marrow cells correlate with disease activity in multicentric Castleman disease‐TAFRO 34873687 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD317Ra01 | Recombinant Glycogen Synthase Kinase 3 Beta (GSK3b) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD317Ra01 | Polyclonal Antibody to Glycogen Synthase Kinase 3 Beta (GSK3b) | WB; IHC; ICC; IP. |
| MAD317Ra21 | Monoclonal Antibody to Glycogen Synthase Kinase 3 Beta (GSK3b) | WB; IHC; ICC; IP. |
| SED317Ra | ELISA Kit for Glycogen Synthase Kinase 3 Beta (GSK3b) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD317Ra | Magnetic Luminex Assay Kit for Glycogen Synthase Kinase 3 Beta (GSK3b) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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