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SKU:LMB452Ge
Magnetic Luminex Assay Kit for Lipoxin A4 (LXA4) ,etc.
Magnetic Luminex Assay Kit for Lipoxin A4 (LXA4) ,etc.
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Product No.
LMB452Ge
Organism Species
Pan-species (General).
Sample Type
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
39.06-40000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 13.02 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex.
Specificity
This assay has high sensitivity and excellent specificity for detection of Lipoxin A4 (LXA4) ,etc..
No significant cross-reactivity or interference between Lipoxin A4 (LXA4) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Lipoxin A4 (LXA4) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Lipoxin A4 (LXA4) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-104 | 99 |
| EDTA plasma(n=5) | 84-98 | 92 |
| heparin plasma(n=5) | 81-101 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipoxin A4 (LXA4) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipoxin A4 (LXA4) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipoxin A4 (LXA4) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-104% | 91-105% | 82-89% | 96-104% |
| EDTA plasma(n=5) | 84-93% | 83-96% | 84-103% | 98-105% |
| heparin plasma(n=5) | 82-99% | 83-98% | 98-105% | 91-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:LXA4) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Neurotoxicology | Redox modulation of cellular stress response and lipoxin A4 expression by Coriolus versicolor in rat brain: Relevance to Alzheimer's disease pathogenesis PubMed: 26433056 |
| Immunity & Ageing | Redox modulation of cellular stress response and lipoxin A4 expression by Hericium Erinaceus in rat brain: relevance to Alzheimer's disease pathogenesis. pubmed:27398086 |
| BMC cardiovascular disorders | Acute coronary syndrome and acute kidney injury: role of inflammation in worsening renal function pubmed:28747177 |
| Liver transplantation | CDP‐choline protects liver from ischemia/reperfusion injury preserving mitochondrial function and reducing oxidative stress Pubmed:29679463 |
| Journal of Immunology | Leukocyte CD300a Contributes to the Resolution of Murine Allergic Inflammation Pubmed: 30315138 |
| Experimental and Therapeutic Medicine | Dynamic changes and clinical significance of LXA4 in the perioperative period of cardiopulmonary bypass |
| Biochem Genet | Lipoxin A4-Mediated p38 MAPK Signaling Pathway Protects Mice Against Collagen-Induced Arthritis 33221976 |
| The mimetic peptide Ac2-26 of annexin A1 attenuates inflammation and apoptosis in sepsis-induced acute kidney injury |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CEB452Ge | ELISA Kit for Lipoxin A4 (LXA4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB452Ge | Magnetic Luminex Assay Kit for Lipoxin A4 (LXA4) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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