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SKU:LMD021Ra
Magnetic Luminex Assay Kit for Ferritin, Heavy Polypeptide (FTH) ,etc.
Magnetic Luminex Assay Kit for Ferritin, Heavy Polypeptide (FTH) ,etc.
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Product No.
LMD021Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.2-200ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.067 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex.
Specificity
This assay has high sensitivity and excellent specificity for detection of Ferritin, Heavy Polypeptide (FTH) ,etc..
No significant cross-reactivity or interference between Ferritin, Heavy Polypeptide (FTH) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Ferritin, Heavy Polypeptide (FTH) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Ferritin, Heavy Polypeptide (FTH) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-95 | 91 |
| EDTA plasma(n=5) | 80-98 | 90 |
| heparin plasma(n=5) | 80-94 | 84 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ferritin, Heavy Polypeptide (FTH) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-89% | 80-90% | 84-101% | 80-96% |
| EDTA plasma(n=5) | 81-90% | 96-105% | 83-97% | 96-104% |
| heparin plasma(n=5) | 81-96% | 93-101% | 80-103% | 88-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:FTH) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| PLoS One | Presence in the Pre-Surgical Fine-Needle Aspiration of Potential Thyroid Biomarkers Previously Identified in the Post-Surgical One PubMed: PMC3765839 |
| Am J Physiol Lung Cell Mol Physiol. | Methemoglobin-induced signaling and chemokine responses in human alveolar epithelial cells Pubmed:24142518 |
| J Alzheimers Dis | The Oral Iron Chelator, Deferasirox, Reverses the Age-Dependent Alterations in Iron and Amyloid-β Homeostasis in Rat Brain: Implications in the Therapy of Alzheimer’s Disease PubMed: 26484920 |
| Eur Radiol | MR molecular imaging of tumours using ferritin heavy chain reporter gene expression mediated by the hTERT promoter Pubmed:26960542 |
| Exp Hematol. | Impact of TET2 deficiency on iron metabolism in erythroblasts. pubmed:28167288 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD021Ra01 | Recombinant Ferritin, Heavy Polypeptide (FTH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD021Ra01 | Polyclonal Antibody to Ferritin, Heavy Polypeptide (FTH) | WB; IHC; ICC; IP. |
| LAD021Ra71 | Biotin-Linked Polyclonal Antibody to Ferritin, Heavy Polypeptide (FTH) | WB; IHC; ICC. |
| MAD021Ra21 | Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) | WB; IHC; ICC; IP. |
| SED021Ra | ELISA Kit for Ferritin, Heavy Polypeptide (FTH) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD021Ra | Magnetic Luminex Assay Kit for Ferritin, Heavy Polypeptide (FTH) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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