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SKU:LMS085Mi
Magnetic Luminex Assay Kit for Angiotensin 1-7 (Ang1-7) ,etc.
Magnetic Luminex Assay Kit for Angiotensin 1-7 (Ang1-7) ,etc.
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Product No.
LMS085Mi
Organism Species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.78-800pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.26 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity for detection of Angiotensin 1-7 (Ang1-7) ,etc..
100% cross-reactivity of Angiotensin 1-7 (Ang1-7) ,etc. was observed among Human, Mouse, Rat.
Recovery
Matrices listed below were spiked with certain level of recombinant Angiotensin 1-7 (Ang1-7) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Angiotensin 1-7 (Ang1-7) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-101 | 96 |
| EDTA plasma(n=5) | 78-94 | 80 |
| heparin plasma(n=5) | 84-96 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin 1-7 (Ang1-7) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin 1-7 (Ang1-7) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin 1-7 (Ang1-7) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-99% | 89-103% | 88-105% | 95-103% |
| EDTA plasma(n=5) | 93-101% | 90-104% | 87-94% | 87-97% |
| heparin plasma(n=5) | 93-102% | 80-101% | 87-96% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:Ang1-7) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| J Stroke Cerebrovasc Dis | Improvement of Plasma Biomarkers after Switching Stroke Patients from Other Angiotensin II Type I Receptor Blockers to Olmesartan PubMed: 25891757 |
| Cellular Physiology and Biochemistry | Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats pubmed:28848201 |
| Frontiers in Physiology | Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression pubmed:29066981 |
| Molecular Medicine Reports | The ACE2-Ang (1-7)-Mas receptor axis attenuates cardiac remodeling and fibrosis in post-myocardial infarction. pubmed:28656296 |
| Journal of Clinical Pharmacy and Therapeutics | Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites. pubmed:28895159 |
| Molecular Medicine Reports | Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats pubmed:28944898 |
| Open Journal of Endocrine and Metabolic Diseases Paper Submission | A Study of the Correlation between Angiotensin (1-7) and the Histopathological Changes in the Penises of Experimentally Diabetic Rats 2-1980272_83198.htm |
| Journal of the American Society of Hypertension | Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system Pubmed:29752040 |
| Innate Immunity | Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis Pubmed:29969931 |
| Evidence-based Complementary and Alternative Medicine | Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects Pubmed:29977313 |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | The impact of ovulation inducing drugs on carcinogenesis |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Changes of angiotensin II (1–8) and angiotensin (1–7) levels in early and late-onset preeclampsia |
| Journals of Gerontology Series A-Biological Sciences and Medical Sciences | Therapeutic Delivery of Ang (1-7) Via Genetically Modified Probiotic: A Dosing Study. Pubmed: 31586210 |
| journal of the european academy of dermatology and venereology | Significance of the angiotensin I/angiotensin II/angiotensin‐(1‐7) axis in the pathogenesis of systemic sclerosis Pubmed: 31746507 |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study Pubmed: 32756181 |
| Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum … | |
| Amino Acids | Angiotensin-(1¨C7) attenuates collagen-induced arthritis via inhibiting oxidative stress in rats 33398523 |
| Autoimmunity | The prevalence and role of functional autoantibodies to angiotensin-converting-enzyme-2 in patients with systemic sclerosis 33910447 |
| researchsquare | Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice |
| Peptides | Angiotensin-(1-7) protects against sepsis-associated left ventricular dysfunction induced by lipopolysaccharide 34298021 |
| BMC Musculoskeletal Disorders | Ang-(1–7) protects skeletal muscle function in aged mice 34548056 |
| J Cardiovasc Pharmacol | Resveratrol Confers Protection Against Ischemia/Reperfusion Injury by Increase of Angiotensin (1-7) Expression in a Rat Model of Myocardial Hypertrophy 34232225 |
| Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| CPS085Mi21 | OVA Conjugated Angiotensin 1-7 (Ang1-7) | Immunogen; SDS-PAGE; WB. |
| CPS085Mi11 | BSA Conjugated Angiotensin 1-7 (Ang1-7) | Immunogen; SDS-PAGE; WB. |
| PAS085Mi08 | Polyclonal Antibody to Angiotensin 1-7 (Ang1-7) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAS085Mi22 | Monoclonal Antibody to Angiotensin 1-7 (Ang1-7) | WB; IHC; ICC; IP. |
| MAS085Mi21 | Monoclonal Antibody to Angiotensin 1-7 (Ang1-7) | WB; IHC; ICC; IP. |
| CES085Mi | ELISA Kit for Angiotensin 1-7 (Ang1-7) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMS085Mi | Magnetic Luminex Assay Kit for Angiotensin 1-7 (Ang1-7) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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